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Related Experiment Videos

Comparing EST-based genetic maps between Pinus sylvestris and Pinus taeda.

P Komulainen1, G R Brown, M Mikkonen

  • 1Department of Biology, University of Oulu, FIN-90014 University of Oulu, Finland.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|June 27, 2003
PubMed
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A new genetic map for Scots pine (Pinus sylvestris) was created using expressed sequence tag polymorphism (ESTP) and other markers. This map facilitates comparisons with related pine species, revealing largely collinear genomes despite ancient divergence.

Area of Science:

  • Genetics and Genomics
  • Plant Science
  • Forestry

Background:

  • Developing high-density genetic maps is crucial for understanding genome structure and evolution in forest trees.
  • Pinus sylvestris (Scots pine) is a commercially important conifer species with a complex genome.
  • Comparative mapping across pine species can reveal conserved genomic regions and evolutionary relationships.

Purpose of the Study:

  • To construct the first genetic map of Pinus sylvestris incorporating expressed sequence tag polymorphism (ESTP) markers.
  • To integrate this map with existing genetic maps of Pinus taeda (loblolly pine) for comparative genomics.
  • To assess the collinearity and evolutionary conservation between Scots pine and loblolly pine genomes.

Main Methods:

  • Construction of an integrated genetic map using 94 F(1) progeny from a cross of Pinus sylvestris parents.

Related Experiment Videos

  • Utilized expressed sequence tag polymorphism (ESTP), amplified fragment length polymorphism (AFLP), and microsatellite markers.
  • Integrated separate maps using shared markers and analyzed segregation patterns for map construction and comparison.

    • Main Results:

    • An integrated genetic map of Pinus sylvestris was developed, comprising 194 AFLP and 61 gene-based markers, totaling 1,314 cM.
    • The map consists of 12 linkage groups corresponding to Pinus taeda groups, plus additional smaller groups.
    • Comparative mapping with Pinus taeda showed significant collinearity between the species' genomes, with successful marker transfer in over 40% of cases.

    Conclusions:

    • The study presents the first ESTP-containing genetic map for Pinus sylvestris, significantly enhancing genomic resources for this species.
    • The largely collinear genomes of Pinus sylvestris and Pinus taeda suggest conserved synteny despite divergence over 70 million years ago.
    • This integrated map serves as a valuable tool for future comparative genomics studies and marker-assisted breeding in pine species.