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Mycobacterium elephantis: not an exceptional finding in clinical specimens
E Tortoli1, L Rindi, A Bartoloni
1Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Piastra dei Servizi, Careggi Hospital, viale Morgagni 85, 50134 Florence, Italy. e.tortoli@libero.it
Summary
This study confirms Mycobacterium elephantis in clinical samples using biochemical, cultural, and genetic methods. A new 16S rDNA sequevar was identified, advancing the understanding of this emerging mycobacterium.
Area of Science:
- Microbiology
- Genetics
- Infectious Diseases
Background:
- Mycobacterium elephantis is a recently identified species.
- Clinical relevance and characterization of M. elephantis are emerging areas of research.
Purpose of the Study:
- To investigate three clinical isolates suspected to be Mycobacterium elephantis.
- To confirm the identity of these strains using multiple methodologies.
- To characterize their antimicrobial susceptibility profile.
Main Methods:
- Biochemical and cultural tests were performed.
- High-performance liquid chromatography (HPLC) of cell wall mycolic acids was utilized.
- Genetic sequencing, specifically 16S rDNA analysis, was conducted.
- Antimicrobial susceptibility testing was determined.
Main Results:
- The findings confirmed the presence of Mycobacterium elephantis in the investigated clinical strains.
- A novel 16S rDNA sequevar for Mycobacterium elephantis was identified.
- The study provides further data on the characteristics of human isolates of this species.
Conclusions:
- The study validates recent reports on Mycobacterium elephantis.
- The identification of a new 16S rDNA sequevar contributes to the genetic diversity understanding of this species.
- This research aids in the accurate identification and characterization of M. elephantis in clinical settings.