Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple method comparison: statistical model using percentage similarity.

Lesley E Scott1, Jacky S Galpin, Deborah K Glencross

  • 1Department of Molecular Medicine and Hematology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. lesleys@mighty.co.za

Cytometry. Part B, Clinical Cytometry
|June 27, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retrospective analysis of CD4 trends at district levels in South Africa between 2013 and 2023.

Southern African journal of HIV medicine·2026
Same author

The impact of a CD4 tiered service model on interlaboratory referral distances in South Africa.

Journal of public health in Africa·2025
Same author

Retrospective analysis of CD4 count trends in South Africa.

Southern African journal of HIV medicine·2025
Same author

Ten-year health impact, economic impact and return on investment of the South African molecular diagnostics programme for HIV, tuberculosis and SARS-CoV-2.

BMJ global health·2024
Same author

Economic impact of extending reflexed cryptococcal antigenaemia CD4 threshold in South Africa.

Southern African journal of HIV medicine·2024
Same author

Assessing very advanced HIV disease in adolescent girls and young women.

Southern African journal of HIV medicine·2023
Same journal

Plasmacytoid dendritic cell proliferation can be clonally related to early T-cell precursor acute lymphoblastic leukemia.

Cytometry. Part B, Clinical cytometry·2026
Same journal

Effects of washing procedures on cell loss in different diagnostic flow cytometry sample types.

Cytometry. Part B, Clinical cytometry·2026
Same journal

What is the optimal threshold for aberrant lymphoblasts at diagnosis to predict lymphoid transformation in chronic myeloid leukemia?

Cytometry. Part B, Clinical cytometry·2026
Same journal

Quantifying interpretive contributions to analytical variability in clinical flow cytometry.

Cytometry. Part B, Clinical cytometry·2026
Same journal

Quantifying gating heterogeneity in the Ogata score: Implications for reproducibility and score-based diagnostics.

Cytometry. Part B, Clinical cytometry·2026
Same journal

DinoFlow: Self-supervised pretraining in flow cytometry enables accurate detection of common hematopathological disorders.

Cytometry. Part B, Clinical cytometry·2026
See all related articles

A new percentage similarity model simplifies method comparison, especially for multiple methods against a gold standard. This approach accommodates wide data ranges and enhances accuracy and precision visualization for quality assessment programs.

Area of Science:

  • Analytical Chemistry
  • Biostatistics
  • Laboratory Medicine

Background:

  • Traditional difference plot models for method comparison struggle with data exhibiting wide absolute value ranges.
  • An alternative model is presented to simplify comparisons, particularly when evaluating multiple methods against a gold standard.

Purpose of the Study:

  • To introduce a novel method comparison model that addresses limitations of traditional approaches.
  • To provide a simplified and versatile tool for assessing agreement between multiple analytical methods and a gold standard.

Main Methods:

  • Calculates percentage similarity by representing the average of a new method and gold standard as a percentage of the gold standard.
  • Utilizes histogram visualization of percentage similarity values to display accuracy and precision.

Related Experiment Videos

  • Incorporates coefficient of variation to further quantify method agreement.

    • Main Results:

    • Percentage similarity histograms allow simultaneous comparison of multiple new methods against a gold standard.
    • A single 100% similarity reference line facilitates easy visualization of comparisons across all plots.
    • The method effectively accommodates and visualizes data with wide ranges of absolute values.

    Conclusions:

    • The proposed percentage similarity method offers a valuable tool for multiple method comparisons.
    • This approach is particularly beneficial for external quality assessment programs to identify inter-laboratory variations.