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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
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Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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Bidirectional expression units enable streptogramin-adjustable gene expression in mammalian cells.

Cornelia Fux1, Martin Fussenegger

  • 1Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.

Biotechnology and Bioengineering
|June 27, 2003
PubMed
Summary

Researchers developed novel bidirectional expression cassettes for gene therapy and tissue engineering. These systems allow tunable co-expression of two transgenes, controlled by clinically approved antibiotics.

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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Biotechnology

Background:

  • Gene therapy and tissue engineering demand advanced molecular tools for precise gene control.
  • Existing systems often lack compactness, robustness, or tunable expression for multiple genes.

Purpose of the Study:

  • To design and validate versatile bidirectional expression cassettes for coadjustable transgene expression.
  • To enable antibiotic-inducible control of gene expression using clinically licensed streptogramins.

Main Methods:

  • Engineered bidirectional expression modules with a central operator (PIR) responsive to a specific transactivator (PIT).
  • Utilized streptogramin antibiotics to regulate PIT binding to PIR, thereby controlling transcription from two divergent promoters.
  • Configured modules for expressing dual effector genes, effector-reporter gene pairs, or a compact one-vector system with transactivator and effector.

Main Results:

  • Successfully designed and implemented streptogramin-adjustable bidirectional expression cassettes.
  • Demonstrated coadjustable expression of two transgenes in various mammalian and human cell lines.
  • Validated configurations for dual gene expression, reporter systems, and compact one-vector arrangements.

Conclusions:

  • The developed bidirectional expression technology offers a sophisticated and versatile molecular toolbox for gene therapy and tissue engineering.
  • Streptogramin-inducible control provides a clinically relevant and tunable method for regulating gene expression.
  • The modular design allows for flexible configurations, enhancing its applicability in diverse research and therapeutic contexts.