Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipids during Bufo arenarum oogenesis.

Ariana Bruzzone1, Jorgelina Buschiazzo, Telma S Alonso

  • 1Instituto de Investigaciones Bioquímicas de Bahía Blanca (UNS-CONICET), C.C. 857, B8000FWB Bahía Blanca, Argentina.

Zygote (Cambridge, England)
|June 28, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Barriers and facilitators in the implementation of the National Colorectal Cancer Prevention Program: a qualitative evaluation from the perspective of health personnel].

Medicina·2025
Same author

Inhibition of phosphodiesterase 4 and 7 regulates breast cancer cell proliferation.

Biochimica et biophysica acta. General subjects·2025
Same author

Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-<i>in vitro</i> matured oocytes subjected to vitrification.

Frontiers in veterinary science·2025
Same author

Quercetin-loaded magnetic nanoparticles: a promising tool for antitumor treatment in human breast cancer cells.

Journal of drug targeting·2025
Same author

[Barriers and facilitators of colorectal cancer screening access].

Medicina·2024
Same author

[Effectiveness of strategies to increase adherence to colorectal cancer screening: a systematic review].

Medicina·2024

Bufo arenarum oocytes show significant changes in phospholipid and triacylglycerol composition during oogenesis. Increased phospholipids, particularly sphingomyelin, and triacylglycerols suggest roles in energy provision and signaling for embryo development.

Area of Science:

  • Reproductive Biology
  • Lipid Biochemistry
  • Developmental Biology

Background:

  • Oogenesis involves complex cellular changes, including lipid metabolism.
  • Phospholipids and triacylglycerols are crucial for cellular structure and energy storage.
  • Understanding lipid dynamics in Bufo arenarum oocytes provides insights into amphibian reproductive strategies.

Purpose of the Study:

  • To investigate the quantitative and qualitative changes in phospholipids and triacylglycerols during Bufo arenarum oogenesis.
  • To identify specific lipid classes and fatty acid profiles in oocytes at stages III and IV.
  • To correlate observed lipid changes with potential functions in oocyte development and energy provision.

Main Methods:

  • Lipid extraction from Bufo arenarum oocytes at stages III and IV.

Related Experiment Videos

  • Analysis of phospholipid and triacylglycerol content using chromatographic techniques.
  • Fatty acid profiling of major lipid classes.

    • Main Results:

    • Total phospholipids increased by 0.5-fold from stage III to IV, with phosphatidylcholine (PC) and phosphatidylethanolamine (PE) as major components.
    • Diphosphatidylglycerol (DPG) was notably high in stage III oocytes, suggesting greater mitochondrial content.
    • Sphingomyelin content increased during oogenesis, potentially linked to signal transduction.
    • Triacylglycerols (TAGs) increased by 0.3-fold from stage III to IV, with significant changes in fatty acid composition, including increased unsaturation in stage IV.
    • PE exhibited higher unsaturation than PC, and both were more unsaturated in stage III oocytes.

    Conclusions:

    • Lipid composition undergoes significant remodeling during Bufo arenarum oogenesis.
    • Increased TAGs likely serve as an energy reserve for embryonic development.
    • Changes in phospholipid profiles, including sphingomyelin and DPG, may reflect developmental stage-specific cellular requirements and signaling roles.