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Updated: Jul 21, 2026

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
Published on: November 7, 2018
Analysis of complement-bound hepatitis B virus complexes by an immuno-capture polymerase chain reaction method
1The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, PR China.
A new immuno-capture PCR (iPCR) method detects Hepatitis B Virus (HBV) complement-bound circulating immune complexes (CIC). This technique aids in understanding HBV immune response and pathogenesis.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Hepatitis B Virus (HBV) circulates as free virus or in immune complexes.
- Complement-bound HBV circulating immune complexes (CIC) in patients are poorly understood.
Purpose of the Study:
- To develop a method for detecting complement-bound HBV CIC.
- To analyze the composition of HBV CIC and understand host immune response.
Main Methods:
- Immuno-capture combined with polymerase chain reaction (iPCR).
- Detection of HBV-C1q-CIC and HBV/factor B-CIC.
Main Results:
- iPCR is a specific and quick method for detecting HBV CIC.
- HBV-factor B frequency correlated with hepatitis B clinical types.
- Increased HBV/C1q-CIC found in patients with normal total bilirubin.
- Increased HBV/factor B-CIC found in HBeAg-positive patients.
Conclusions:
- iPCR is valuable for analyzing immune complex composition in HBV infection.
- This method offers insights into HBV immune pathogenesis and host response.
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