Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Drosophila PS2 integrin mediates RGD-dependent cell-matrix interactions.

T A Bunch1, D L Brower

  • 1Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.

Development (Cambridge, England)
|September 1, 1992
PubMed
Summary

Drosophila PS2 integrin, a cell adhesion molecule, mediates cell spreading on various substrates. This function is dependent on divalent cations and can be blocked by RGD peptides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of Nonlinear Coupling in the Edge Harmonic Oscillation Sustaining Quiescent High Confinement in a Tokamak Plasma.

Physical review letters·2026
Same author

Upgrade of DIII-D radial interferometer-polarimeter for large bandwidth, low noise, and toroidal mode number measurements.

The Review of scientific instruments·2024
Same author

Design of radial interferometer-polarimeter for internal magnetic and density fluctuation measurements at multiple space-time scales in the National Spherical Torus Experiment-Upgrade (NSTX-U).

The Review of scientific instruments·2024
Same author

Implementing Faraday effect measurement constraints into the Grad-Shafranov equilibrium fitting code EFIT.

The Review of scientific instruments·2023
Same author

Non-inductive plasma vertical position measurement for the 1056 s discharge on EAST.

The Review of scientific instruments·2022
Same author

Faraday-effect polarimetry for current profile measurement in the tokamak plasma edge.

The Review of scientific instruments·2022

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Integrins are crucial transmembrane glycoproteins involved in cell adhesion.
  • They mediate interactions between cells and the extracellular matrix, as well as cell-cell adhesion.
  • Understanding integrin function is key to deciphering cellular communication and tissue development.

Purpose of the Study:

  • To investigate the functional role of the Drosophila PS2 integrin in cultured cells.
  • To determine the substrates and conditions under which PS2 integrin mediates cell adhesion and spreading.
  • To explore the molecular mechanisms, including cation dependence and peptide inhibition, of PS2 integrin-mediated cell spreading.

Main Methods:

  • Transfection of cultured Drosophila cells with genes encoding Drosophila PS2 integrin.
  • Assessing integrin expression on the cell surface.
  • Conducting cell spreading assays on various substrates like fetal calf serum, vitronectin, fibronectin, and RGD peptide.
  • Investigating the effects of soluble RGD peptide and divalent cations on cell spreading.

Main Results:

  • Drosophila PS2 integrin was successfully expressed on the surface of transfected cells.
  • The expressed PS2 integrin mediated significant cell spreading on fetal calf serum, vitronectin, and fibronectin.
  • Cell spreading was also observed on RGD peptide, indicating specific molecular interactions.
  • Soluble RGD peptide inhibited PS2 integrin-mediated spreading, and the process was dependent on divalent cations.

Conclusions:

  • Drosophila PS2 integrin functions as a cell adhesion receptor capable of mediating cell spreading.
  • Its activity is regulated by specific matrix components and molecular cues like RGD sequences.
  • The cation dependence and RGD inhibition highlight conserved mechanisms of integrin signaling across species.

Related Experiment Videos