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Updated: Sep 23, 2026
Measurement of Nitric Oxide Production in Mouse Tracheal Epithelial Cell Cultures Differentiated at the Air Liquid Interface
Published on: July 3, 2026
Release of nitric oxide by N-nitropyrazoles in rabbit lacrimal gland cell culture
1Institute of Ocular Pharmacology and Department of Medical Pharmacology and Toxicology, Texas A & M University System Health Science Center, College Station 77843, USA.
Purpose:
In order to prove the effects of N-nitropyrazoles to increase ocular blood flow and to facilitate retinal function after ischemia are due to nitric oxide (NO) release, eleven N-nitropyrazoles which are known NO donors were used to test their ability to produce NO in rabbit lacrimal gland (RLG) cell cultures.
Methods:
Nitropyrazoles (0.3-3 microg/ml) were added into RLG cell culture and the NO produced was determined with nitrate-nitrite colorimetric assay.
Results:
All nitropyrazoles (11 compounds) tested produced NO in various degrees with the highest degree by 13 fold over control (DN-5) and the lowest level by only 2.8 fold over control (DN-7).
Conclusion:
The results indicated that NO is involved in ocular blood flow increase and retinal function recovery after ischemia by N-nitropyrazoles. However, there is no potency relationship between in vitro data and in vivo data, which is understandable because the experimental environments are different.
