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Updated: Aug 15, 2026
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 10, 2009
Virus-induced gene silencing in plants
Rui Lu1, Ana Montserrat Martin-Hernandez, Jack R Peart
1The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Methods (San Diego, Calif.)
|June 28, 2003
Summary
Virus-induced gene silencing (VIGS) uses plant defense mechanisms to study gene function. This technology is adaptable for high-throughput genomics and identifying genes involved in plant disease resistance.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Virus-induced gene silencing (VIGS) leverages RNA-mediated antiviral defense in plants.
- VIGS targets viral genomes but can be adapted to silence host gene mRNAs using virus vectors.
Purpose of the Study:
- To discuss practical and theoretical aspects of VIGS for gene function analysis.
- To present protocols for applying VIGS in identifying genes essential for plant disease resistance.
Main Methods:
- Utilizing virus vectors carrying host gene inserts to trigger gene silencing.
- Adapting VIGS technology for high-throughput functional genomics studies.
- Developing new vector systems for VIGS applications in diverse plant species, including Arabidopsis.
Main Results:
- VIGS enables targeted gene "knock down" for functional analysis.
- Protocols are described for VIGS application in identifying disease resistance genes.
- The principles of VIGS are applicable to various aspects of plant biology.
Conclusions:
- VIGS is a versatile tool for plant gene function analysis and functional genomics.
- The technology is expanding beyond model organisms like Nicotiana benthamiana.
- VIGS facilitates the identification of genes critical for plant traits, such as disease resistance.
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