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Updated: Sep 23, 2026

An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
Published on: March 10, 2023
Sensitive determination of pethidine in body fluids by gas chromatography-tandem mass spectrometry
Akira Ishii1, Miwa Tanaka, Rina Kurihara
1Department of Legal Medicine, Fujita Health University School of Medicine, 1-98 Kutsukake-cho, Toyoake 470-1192, Japan. akishii@fujita-hu.ac.jp
Abstract:
We have presented a simple and sensitive method for determining pethidine, a narcotic analgesic drug in body fluids by gas chromatography-tandem mass spectrometry (GC-MS/MS). Pethidine and 4'-piperidinoacetophenone (internal standard) were extracted from body fluids with Bond Elut C(18) columns; the recoveries were above 85% for both compounds. The calibration curves for blood and urine showed good linearities in the range of 1.25-40 ng/ml. Its detection limits (signal-to-noise ratio=3) were estimated to be approximately 0.5 ng/ml of whole blood and urine.
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