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Updated: Sep 23, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
Unravelling the activation mechanisms of protein kinase B/Akt
Michael P Scheid1, James R Woodgett
1Ontario Cancer Institute, 610 University Avenue, Toronto, ON, Canada M5G 2M9.
Abstract:
Over the past decade, protein kinase B (PKB, also termed Akt) has emerged as an important signaling mediator between extracellular cues and modulation of gene expression, metabolism, and cell survival. The enzyme is tightly controlled and consequences of its deregulation include loss of growth control and oncogenesis. Recent work has better characterized the mechanism of PKB activation, including upstream regulators and secondary binding partners. This minireview refreshes some old concepts with new twists and highlights current outstanding questions.
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