Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The frameshift infidelity of human DNA polymerase lambda. Implications for function.

Katarzyna Bebenek1, Miguel Garcia-Diaz, Luis Blanco

  • 1Laboratory of Molecular Genetics and Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA.

The Journal of Biological Chemistry
|June 28, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Persistence of large mtDNA rearrangements linked to premature aging in Pol γ exonuclease-deficient mice.

Nucleic acids research·2026
Same author

Evidence that MutSβ repairs indels generated by mispair initiated template slippage.

DNA repair·2026
Same author

Nonhomologous end-joining uses distinct mechanisms to repair each strand of a double strand break.

Nature communications·2025
Same author

Novel primer-less amplification method for detection of RNA molecules.

Methods (San Diego, Calif.)·2025
Same author

Mitochondrial Transcription: A click-chemistry derived detection methodology forgoing the use of radiation in in vitro analyses.

Mitochondrion·2025
Same author

A novel technology for margin extension and local control in breast conservation surgery: Saline-coupled intraoperative radiofrequency ablation (SIRA).

Surgical oncology·2025
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

Human DNA polymerase lambda (Pol lambda) shows a high rate of single-base deletions, exceeding substitution rates. This suggests a role in DNA repair pathways like non-homologous end-joining.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA polymerase lambda (Pol lambda) belongs to the Pol X family of enzymes.
  • Mammalian DNA polymerases share common properties and functions.
  • Understanding Pol lambda's in vivo functions requires fidelity studies.

Purpose of the Study:

  • To determine the fidelity of DNA synthesis by human Pol lambda.
  • To investigate the potential in vivo functions of Pol lambda.

Main Methods:

  • Assessed the fidelity of DNA synthesis by human Pol lambda.
  • Analyzed error rates, specifically single-base deletions and base substitutions.
  • Examined sequence specificity for deletions and primer-template homology utilization.

Related Experiment Videos

Main Results:

  • Human Pol lambda exhibits a significantly higher average single-base deletion error rate compared to other mammalian DNA polymerases.
  • Pol lambda's deletion error rates surpass its base substitution rates.
  • Pol lambda demonstrates a distinct sequence specificity for single-base deletions and efficiently uses template-primers with limited terminal homology.

Conclusions:

  • Pol lambda's high deletion error rate and ability to utilize mismatched primer termini suggest a role in non-homologous end-joining (NHEJ).
  • Its capacity to fill short DNA gaps at low dNTP concentrations further supports a role in NHEJ.
  • Intrinsic 5',2'-deoxyribose-5-phosphate lyase activity in Pol lambda may contribute to its function in repairing DNA strand breaks from damage.