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[Ginsenoside Rg1 may protect SHSY5Y cells from apoptosis induced by MPP+ through JNK way]
Fang Fang1, Xiao-chun Chen, Yuan-gui Zhu
1Fujian Institute of Geriatrics, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Aim:
To explore possible signal transmission way through which ginsenoside Rg1 protect cells from MPP(+)-induced apoptosis.
Methods:
The apoptosis of SHSY5Y induced by 1-methyl-4-phenylpyridinium (MPP+) was observed by AO-EB staining. Flow cytometry was used to quantitate the reactive oxygen species (ROS). Western Blotting was used to detect the c-jun NH2-terminal kinase (JNK) activity in SHSY5Y cells. Immunocytochemistry staining was used to detect cleaved Caspase-3 positive cells.
Results:
MPP+ was shown to induce apoptosis in SHSY5Y cells. The percentage of apoptotic SHSY5Y cells induced by MPP+ was obviously lower in those groups pretreated with 10 mumol.L-1 Rg1 or 2.5 mmol.L-1 N-acetylcysyteine (NAC). It showed more ROS in MPP+ groups than in control. JNK activity increased with time within 72 hours in 1 mmol.L-1 MPP+ group. Simultaneously, it showed decrease of ROS, less activity of JNK and lower expression of cleaved Caspase-3 in 10 mumol.L-1 Rg1 and 2.5 mmol.L-1 NAC pretreated groups compared with groups treated with MPP+ only.
Conclusion:
Rg1 protects against MPP(+)-induced apoptosis in SHSY5Y cells and the effect might be attributed to its removal of ROS, inhibition of the activity of JNK and expression of cleaved Caspase-3.
Insights
Ginsenoside Rg1 protects SHSY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced apoptosis. This neuroprotection involves reducing reactive oxygen species (ROS), inhibiting c-jun NH2-terminal kinase (JNK) activity, and decreasing cleaved Caspase-3 expression.
Area of Science:
- Neuroscience
- Pharmacology
- Cell Biology
Background:
- MPP+ induces apoptosis in SHSY5Y cells, a model for Parkinson's disease.
- Ginsenoside Rg1 is a compound with potential neuroprotective properties.
Purpose of the Study:
- To investigate the mechanism by which ginsenoside Rg1 protects against MPP+-induced apoptosis.
- To explore the role of reactive oxygen species (ROS) and c-jun NH2-terminal kinase (JNK) signaling in this protective effect.
Main Methods:
- Apoptosis was assessed using AO-EB staining and flow cytometry.
- ROS levels were quantified by flow cytometry.
- JNK activity and cleaved Caspase-3 expression were detected using Western Blotting and immunocytochemistry, respectively.
Main Results:
- MPP+ treatment significantly increased apoptosis, ROS levels, JNK activity, and cleaved Caspase-3 expression in SHSY5Y cells.
- Pretreatment with ginsenoside Rg1 (10 µmol/L) or N-acetylcysteine (NAC, 2.5 mmol/L) attenuated MPP+-induced apoptosis.
- Rg1 and NAC pretreatment reduced ROS levels, JNK activity, and cleaved Caspase-3 expression compared to MPP+ alone.
Conclusions:
- Ginsenoside Rg1 exhibits neuroprotective effects against MPP+-induced apoptosis in SHSY5Y cells.
- The protective mechanism is associated with the reduction of ROS, inhibition of JNK signaling pathway, and decreased cleaved Caspase-3 expression.
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