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Updated: Sep 21, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Isolation and structure identification of flavonol glycoside from Lysimachia christinae Hance]
Dong-bin Cui1, Shu-qin Wang, Ming-ming Yan
1Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province, Changchun 130021, China. cc_cdb@sohu.com
Aim:
To isolate and prepare chemical standards.
Methods:
Flavonol glycoside and rhamnonic acid gamma-lactone were isolated from whole plants of Lysimachia christinae Hance. with 95% ethanol and purified by silica gel column chromatography. UV, IR, MS, 1HNMR, 13CNMR, DEPT, HMQC, HMBC were used for the structural identification.
Results:
Two compounds were obtained. They were identified as rhamnonic acid gamma-lactone (I) and kaempferol-3-O-alpha-L-rhamnosyl (1-->2)-beta-D-xyloside (II).
Conclusion:
Compound I was isolated from this genus for the first time. Compound II is a new compound named as lysimachiin.

