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Mass transfer in rectangular chromatographic channels.

H Poppe1

  • 1University of Amsterdam, Institute of Technical Chemistry, Research Group Polymer Analysis, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands. poppe@anal.chem.uva.nl

Journal of Chromatography. A
|July 2, 2003
PubMed
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This study modifies plate height equations for rectangular channels used in separations, accounting for finite width and electroosmotic flow. Finite channel width significantly impacts unretained solutes but has minor effects on retained ones.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Rectangular channels are increasingly used in microfluidic separations.
  • Existing plate height equations by Giddings and Golay primarily address infinite or specific finite channel widths and unretained analytes.
  • Modifications are needed for retained analytes and electroosmotic flow in finite rectangular channels.

Purpose of the Study:

  • To develop and present modified plate height equations for rectangular channels.
  • To investigate the impact of finite channel width on chromatographic and electrophoretic separations.
  • To account for electroosmotic flow profiles in plate height calculations.

Main Methods:

  • Numerical Fourier transform approach was employed.

Related Experiment Videos

  • Plate height contributions from mobile phase mass transfer were analyzed.
  • Results were compared to the established Golay equation for cylindrical systems.

    • Main Results:

    • New expressions for plate height (H) were derived, incorporating channel width-to-height ratio (phi).
    • The finite channel width significantly increases plate height for unretained solutes (up to eightfold).
    • The effect of finite width on retained solutes is generally minor, with retention factor (k') playing a key role.

    Conclusions:

    • The study provides essential equations for optimizing rectangular channel-based separations.
    • Finite channel width effects are substantial for unretained analytes, necessitating adjustments to theoretical models.
    • The findings are applicable to various channel coatings and flow conditions, including electroosmotic flow.