Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Advanced high-performance liquid chromatography method development. Discovering unexpected choices in chromatography.

H J Rieger1, I Molnar

  • 1Institut für Angewandte Chromatographie, Schneeglöckchenstrasse 47, D-10407 Berlin, Germany.

Journal of Chromatography. A
|July 2, 2003
PubMed
Summary

Understanding experimental parameters is key to improving compound resolution. Resolution maps reveal all chances for increasing selectivity, going beyond common expectations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Calling in assistants in an official expert opinion at court].

Deutsche medizinische Wochenschrift (1946)·2011
Same author

[Breach of confidential medical communication and personal rights as a result of a medical certificate from a tenured physician].

Deutsche medizinische Wochenschrift (1946)·2011
Same author

[Consenting patients by phone].

Deutsche medizinische Wochenschrift (1946)·2010
Same author

[Amended judgment of malpractice due to delayed expert testimony. Decision by the German Federal Court 22 November 2005].

Deutsche medizinische Wochenschrift (1946)·2006
Same author

[Increased compensation for legal experts. Decision of the Lower Saxony-Bremen Federal Social Court 27 January 2003--L 4 SF 17/02].

Deutsche medizinische Wochenschrift (1946)·2003
Same author

[Liability of the physician in the refusal of a clinic admission by the patient. Decision of the Provincial High Court of Zweibrücken from August 20, 2002--5 U 25/01].

Deutsche medizinische Wochenschrift (1946)·2003

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Optimizing compound resolution in analytical methods is crucial for accurate analysis.
  • Commonly used rules of thumb for improving resolution may not encompass all possibilities.

Purpose of the Study:

  • To discuss the influence of experimental parameters on compound resolution.
  • To evaluate the validity of common expectations for resolution improvement.
  • To introduce the resolution map as a tool for enhancing selectivity.

Main Methods:

  • Discussion of experimental parameters affecting compound resolution.
  • Analysis of selected examples to illustrate resolution improvement strategies.
  • Utilizing resolution maps to understand and enhance method selectivity.

Related Experiment Videos

Main Results:

  • General expectations for parameter adjustment do not always lead to optimal resolution.
  • Selected examples demonstrate that all chances for resolution improvement are not commonly covered.
  • Resolution maps provide a comprehensive understanding of method selectivity.

Conclusions:

  • Experimental parameter optimization requires a deeper understanding beyond common rules.
  • Resolution maps are powerful tools for maximizing compound separation and analytical method performance.