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High-performance liquid chromatographic stationary phases based on poly(methyloctylsiloxane) immobilized on silica.
Edivan Tonhi1, Stefan Bachmann, Klaus Albert
1Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13083-970 Campinas, SP, Brazil.
Abstract:
Five different reversed-phase materials for high-performance liquid chromatography were obtained by deposition of poly(methyloctylsiloxane) in HPLC silica particles, followed by immobilization using different processes: thermal treatment (120 or 220 degrees C for 4 h), irradiation with microwaves (495 W for 15 min), gamma radiation (dose of 80 kGy) and self-immobilization. These phases were characterized by gel permeation chromatography, percent carbon, 13C and 29Si solid-state nuclear magnetic resonance spectroscopy, infrared spectroscopy, thermogravimetric analysis and scanning electron microscopy. The results show that the different immobilization processes produce different physical characteristics in the prepared phases.
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