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Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit

W Nastainczyk1, O-G Issinger, B Guerra

  • 1Medizinische Biochemie und Molekularbiologie der Universitaet des Saarlandes, 66421 Homburg, Germany.

Hybridoma and Hybridomics
|July 2, 2003
PubMed

1AD9 is a murine monoclonal antibody (MAb) that was produced against the recombinant human alpha-subunit of protein kinase CK2, a pleiotropic and constitutively active serine/threonine protein kinase. We have further characterized this antibody, which is suitable for Western blot, immunoprecipitation and enzyme-linked immunosorbant assay tests. Using an overlapping peptide library, we have identified the epitope targeted by MAb1AD9 characterized by the following sequence: (319)MEHPYF(324).

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