Related Experiment Video
Updated: Aug 19, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Generation and characterization of species-specific anti-Bcl-X(L) monoclonal antibodies
Yi-Te Hsu1, Qi Hou, Eugene Cymbalyuk
1Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA. hsu@musc.edu
Abstract:
Bcl-X(L) is a pro-survival member of the Bcl-2 family that plays an important role in apoptosis regulation. As a first step to carry out the molecular characterization of this protein, we have generated five monoclonal antibodies (MAbs) directed against synthetic peptides corresponding to the flexible loop region of human and murine Bcl-X(L). These antibodies display species specificity and are suitable for Western blotting, immunofluorescence labeling, and immunoprecipitation. These results suggest that these antibodies can serve as important tools for the detection and purification of endogenous and ectopically expressed human and murine Bcl-X(L).
Related Concept Videos
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

