Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

506
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
506
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

580
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
580
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.0K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.0K
DNA Microarrays02:34

DNA Microarrays

20.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Features and Pervasive Negative Selection in <i>Rhodanobacter</i> Strains Isolated from Nitrate and Heavy Metal Contaminated Aquifer.

Microbiology spectrum·2022
Same author

Long-Term Effects of Soil Remediation with Willow Short Rotation Coppice on Biogeographic Pattern of Microbial Functional Genes.

Microorganisms·2022
Same author

Nutrient supply controls the linkage between species abundance and ecological interactions in marine bacterial communities.

Nature communications·2022
Same author

Disentangling direct from indirect relationships in association networks.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Soil enzymes as indicators of soil function: A step toward greater realism in microbial ecological modeling.

Global change biology·2021
Same author

Permafrost thaw with warming reduces microbial metabolic capacities in subsurface soils.

Molecular ecology·2021

Related Experiment Video

Updated: Jan 15, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
06:27

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

517

Microarrays for bacterial detection and microbial community analysis.

Jizhong Zhou1

  • 1Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831-6038, USA. zhouj@ornl.gov

Current Opinion in Microbiology
|July 2, 2003
PubMed
Summary
This summary is machine-generated.

Microarrays offer specific, sensitive bacterial detection and community analysis. However, reliable single-base detection requires further development for microbial applications.

More Related Videos

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.6K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

Related Experiment Videos

Last Updated: Jan 15, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
06:27

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

517
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.6K
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bioinformatics

Background:

  • Microarrays are increasingly used for bacterial detection and microbial community analysis.
  • Both functional gene arrays and community genome arrays show promise for specific, sensitive, and quantitative detection.

Purpose of the Study:

  • To evaluate the capabilities of various microarray types for bacterial detection and microbial community analysis.
  • To identify limitations and areas for improvement in current microarray technologies for microbial applications.

Main Methods:

  • Review and evaluation of studies utilizing functional gene arrays, community genome arrays, and phylogenetic oligonucleotide arrays.
  • Assessment of hybridization approaches for genome diversity and bacterial relatedness analysis.

Main Results:

  • Microarrays demonstrate specific, sensitive, and quantitative detection of bacteria and microbial communities.
  • Phylogenetic oligonucleotide arrays can differentiate single-base mismatches, but reliable single-base specificity remains a challenge.
  • Microarray hybridization is effective for analyzing genome diversity and bacterial relationships.

Conclusions:

  • Microarrays are valuable tools for microbial detection and community analysis, offering specificity and sensitivity.
  • Further rigorous development is necessary to overcome limitations in single-base detection and fully exploit microarray potential.
  • Continued research is essential for advancing microarray applications in microbiology.