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Molecular cloning, nucleotide sequence and expression of a cDNA encoding an intracellular protein tyrosine
1Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Abstract:
The PTP-2 cDNA encoding an intracellular protein tyrosine phosphatase (PTPase-2) was isolated and sequenced from mouse testis and T-cell cDNA libraries. This PTP-2 cDNA was found to be homologous to human PTP-TC and rat PTP-S, and contained 1,551 nucleotides, including 1,146 nucleotides encoding 382 amino acids as well as 5' (61 nucleotides) and 3' (344 nucleotides) non-coding regions. Northern blot analysis indicated that PTP-2 mRNA of 1.9 Kb was most abundant in testis and kidney, although it was also present in spleen, muscle, liver, heart and brain.
Insights
Researchers isolated and sequenced the PTP-2 cDNA, identifying its homology to other phosphatases. PTP-2 mRNA is most abundant in mouse testis and kidney, suggesting tissue-specific roles.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Intracellular protein tyrosine phosphatases (PTPases) play critical roles in cellular signaling pathways.
- Understanding the diversity and tissue distribution of PTPases is essential for elucidating their functions.
Purpose of the Study:
- To isolate and sequence the complementary DNA (cDNA) encoding the mouse intracellular protein tyrosine phosphatase PTP-2.
- To investigate the homology and expression patterns of PTP-2.
Main Methods:
- cDNA library screening and sequencing from mouse testis and T-cell libraries.
- Northern blot analysis to determine PTP-2 mRNA expression levels in various mouse tissues.
Main Results:
- The PTP-2 cDNA was successfully isolated and sequenced, revealing a coding sequence of 1,146 nucleotides encoding 382 amino acids.
- PTP-2 cDNA exhibited homology to human PTP-TC and rat PTP-S.
- Northern blot analysis detected a 1.9 Kb PTP-2 mRNA transcript, with highest abundance in testis and kidney, and lower levels in spleen, muscle, liver, heart, and brain.
Conclusions:
- The PTP-2 gene encodes a protein tyrosine phosphatase with conserved domains homologous to known phosphatases.
- The distinct expression pattern of PTP-2 mRNA, particularly its high levels in testis and kidney, suggests specialized functions in these tissues.
