Macroarray analysis of the effects of JP-8 jet fuel on gene expression in Jurkat cells

The jet fuel JP-8 is widely used and a large number of military and civilian personnel is, therefore, exposed to it. Treatment of several cell lines, including human Jurkat cells, with JP-8 induces cell death that exhibits various biochemical and morphological characteristics of apoptosis. The molecular mechanism of JP-8 cytotoxicity, however, has remained unclear. The effects of exposure of Jurkat cells to JP-8 (1/10,000 dilution) for 4 h on gene expression have now been examined by cDNA macroarray analysis. We had previously shown in these cells that under the above conditions, JP-8 causes significant apoptosis, based upon the observation that caspase-3 activation occurs at approximately 4 h and consequently most of the other classical apoptotic biochemical and morphological alterations progress until apoptotic cell death at 24 h. Of the 439 apoptosis- or stress response-related genes examined, the expression of 16 genes was up-regulated and that of ten genes was down-regulated by a factor of > or =2. The changes in the expression of 11 of these 26 genes were confirmed by reverse transcription and polymerase chain reaction analysis. These results provide insight into the mechanism of JP-8 toxicity and the associated induction of apoptosis.