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Mouse mammary epithelial histamine system.

W Wagner1, A Ichikawa, S Tanaka

  • 1Institute of Biogenic Amines, Polish Academy of Sciences, Lodz, Poland. waldemaw@amina1.zabpan.lodz.pl

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
|July 2, 2003
PubMed
Summary
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Mammary epithelial cells synthesize histamine, crucial for mammary gland growth and function during pregnancy and lactation. These cells express histamine receptors, supporting its physiological role.

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Biochemistry

Background:

  • Histamine is implicated in mammary gland development and function.
  • Two histamine pools exist: mast cell- and epithelial cell-derived.
  • This study investigates the role of histamine in mammary epithelial cells.

Purpose of the Study:

  • To explore histamine synthesis and receptor presence in mammary epithelial cells.
  • To understand histamine's role in mammary gland physiology.
  • To investigate histamine's regulation during pregnancy and lactation.

Main Methods:

  • Immunohistochemistry to detect histamine and L-histidine decarboxylase (HDC).
  • Culture of primary mouse mammary epithelial cells (MMEC).
  • Histamine receptor binding studies on mammary tissue and epithelial cell membranes.

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Main Results:

  • Epithelial cells in mammary alveolar structures express histamine and HDC.
  • MMEC show strong HDC immunoreactivity, particularly in dividing cells.
  • HDC expression changes during pregnancy (increase) and lactation (decrease).
  • H1 and H3 histamine receptors are present, but H2 receptors are not.

Conclusions:

  • Mammary epithelial cells synthesize and excrete histamine.
  • These cells possess histamine receptors (H1 and H3).
  • Findings support histamine's significant role in mammary gland physiology.