Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mining single-nucleotide polymorphisms from hexaploid wheat ESTs.

Daryl J Somers1, Robert Kirkpatrick, Mariko Moniwa

  • 1Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Road, Winnipeg, MB R3T 2M9, Canada. somersd@agr.gc.ca

Genome
|July 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of Covalent 6-Cyanoquinazoline KRAS<sup>G12C</sup> Inhibitors for the Treatment of Solid Tumors.

Journal of medicinal chemistry·2026
Same author

From Classroom to Practice: Boosting ENP Proficiency With Online POCUS Training.

Advanced emergency nursing journal·2025
Same author

Genetic and Environmental Influences on Fear Learning and Generalization.

Psychophysiology·2025
Same author

Impact of social vulnerability index on patients with alcohol-related liver disease.

Alcohol (Fayetteville, N.Y.)·2024
Same author

Preclinical efficacy of the potent, selective menin-KMT2A inhibitor JNJ-75276617 (bleximenib) in KMT2A- and NPM1-altered leukemias.

Blood·2024
Same author

Call for action: addressing the alarming surge of HIV in Madagascar.

BMJ global health·2024
Same journal

Two satellite repeats reveal B chromosome structural diversity in Atractylodes lancea.

Genome·2026
Same journal

A Drosophila teissieri I-element retrotransposon's ORF1p shows RNA binding cis-preference in the D. melanogaster female germline.

Genome·2026
Same journal

Abiotic stress-responsive tRNA-derived fragments in pitanga (<i>Eugenia uniflora</i> L.): regulatory roles in drought and salinity adaptation.

Genome·2026
Same journal

Diacylglycerol kinase promotes forgetting of aversive olfactory memory in Drosophila.

Genome·2026
Same journal

From Lab Bench to Public Voice: How to Incorporate Science Communication into Your Research Program.

Genome·2026
Same journal

<i>delimtools</i>: an R package for species delimitation.

Genome·2026
See all related articles

Researchers discovered single-nucleotide polymorphisms (SNPs) in wheat expressed sequence tags (ESTs) using bioinformatics. These SNPs are valuable for wheat genetic mapping and understanding genetic diversity.

Area of Science:

  • Plant genetics
  • Bioinformatics
  • Molecular biology

Background:

  • Single-nucleotide polymorphisms (SNPs) are functional markers, especially when derived from expressed sequence tags (ESTs).
  • Wheat (Triticum aestivum) genetic studies require efficient and abundant marker systems.

Purpose of the Study:

  • To develop a bioinformatics strategy for discovering SNPs within a large wheat EST database.
  • To demonstrate the utility of these SNPs in wheat genetic mapping and genetic diversity analyses.

Main Methods:

  • Assembled over 90,000 wheat ESTs into contiguous sequences (contigs).
  • Visually inspected 45 random contigs to identify primer pairs for allele amplification.
  • Estimated SNP frequency and polymorphism information content (PIC).

Related Experiment Videos

Main Results:

  • Identified a high frequency of homoeologue sequence variants (1 in 24 bp) and SNPs between wheat genotypes (1 SNP/540 bp).
  • Estimated that one diagnostic SNP can be developed from contigs with 10-60 EST members, indicating EST databases are rich in SNP markers.
  • Determined SNP PIC values ranging from 0.04 to 0.50.
  • Successfully mapped EST-derived SNPs into a microsatellite marker framework using segregating populations.

Conclusions:

  • EST databases are a valuable and abundant source for discovering and validating SNP markers in wheat.
  • Developed SNPs are effective for wheat genetic mapping and assessing genetic diversity.
  • This bioinformatics approach facilitates the application of SNPs in conventional genetic studies.