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A modified mordant technique for staining plant chromosomes
T J Kubik1, G P Hawkins, G R Stringam
1Dow AgroSciences Canada Inc., 101-421 Downey Road, Saskatoon, SK S7N 4L8, Canada.
Genome
|July 2, 2003
Summary
Researchers developed an improved method for creating ferric acetate, a key component in Newcomer's fluid. This innovation offers a new mordant source for analyzing small chromosomes, enhancing previous techniques.
Area of Science:
- Cytogenetics
- Histotechnology
- Biochemistry
Background:
- Newcomer's fluid is a histological fixative used in cytogenetics.
- A previously established method for preparing a key component of Newcomer's fluid was published in 1970.
- Access to reliable mordant sources is crucial for chromosome analysis.
Purpose of the Study:
- To develop an improved in-house method for producing ferric acetate.
- To establish a new mordant source for Newcomer's fluid.
- To enhance the analysis of species with small chromosomes.
Main Methods:
- In-house synthesis of ferric acetate.
- Preparation of Newcomer's fluid utilizing the synthesized ferric acetate.
- Application of the modified Newcomer's fluid for chromosome preparation.
Main Results:
- Successful in-house production of ferric acetate.
- Ferric acetate serves as an effective mordant in Newcomer's fluid.
- The improved method facilitates the analysis of small chromosomes.
Conclusions:
- The in-house production of ferric acetate provides a valuable new mordant source.
- This updated method enhances the utility of Newcomer's fluid for cytogenetic studies.
- The technique offers an improvement over the original 1970s method.