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Escherichia coli can be transformed by a liposome-mediated lipofection method
Yoshikazu Kawata1, Shin-ichi Yano, Hiroyuki Kojima
1Special Division for Green Life Technology, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. y-kawata@aist.go.jp
Bioscience, Biotechnology, and Biochemistry
|July 2, 2003
Abstract:
Transformation of Escherichia coli is a basic technique for genetic engineering. We used a liposome-mediated lipofection method to transform electrocompetent E. coli cells which has little natural competence of foreign DNA without electroporation treatment, and got transformants with simple and quick treatment by a plasmid or a transposon and transposase complex.