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Related Experiment Videos

Interactions between pH-sensitive liposomes and model membranes.

Nill Bergstrand1, Maria C Arfvidsson, Jong-Mok Kim

  • 1Department of Physical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden. nill.bergstrand@fki.uu.se

Biophysical Chemistry
|July 2, 2003
PubMed
Summary

pH-sensitive liposomes containing dioleoylphosphatidylethanolamine (DOPE) do not fuse with endosomes. Instead, DOPE incorporation destabilizes the endosome membrane, facilitating cytoplasmic delivery.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Drug Delivery

Background:

  • pH-sensitive liposomes are crucial for targeted drug delivery.
  • Understanding liposome-endosome interactions is key to optimizing delivery efficiency.
  • Dioleyolphosphatidylethanolamine (DOPE) is a common component in liposome formulations.

Purpose of the Study:

  • To investigate the structure and dynamics of two pH-sensitive liposome systems.
  • To elucidate the mechanism of interaction between DOPE-containing liposomes and endosome models.
  • To determine if direct fusion occurs between liposomes and endosomes.

Main Methods:

  • Cryo-transmission electron microscopy
  • Photophysical techniques (proton-induced leakage, lipid mixing)

Related Experiment Videos

  • Liposome preparation with dioleoylphosphatidylethanolamine (DOPE) and stabilizers (oleic acid or DHCho-MPEG5000)
  • Interaction studies with EPC liposomes and endosome-like liposomes

    • Main Results:

    • Neither oleic acid- nor DHCho-MPEG5000-stabilized liposomes showed fusion with EPC or endosome-like liposomes.
    • Incorporation of pH-sensitive liposome lipids into the endosome membrane increased permeability.
    • Formation of non-lamellar structures was observed upon lipid incorporation.

    Conclusions:

    • Direct, non-leaky fusion between DOPE-containing liposomes and endosomes is not the mechanism for cytoplasmic delivery.
    • Lipid incorporation and subsequent destabilization of the endosome membrane is a more plausible mechanism.
    • This study provides insights into the non-fusogenic mechanisms of liposome-mediated delivery.