Related Experiment Video
Updated: Sep 23, 2026

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Published on: January 18, 2019
The activation of 5-HT(3) receptors evokes GABA release in the spinal cord
Tomoyuki Kawamata1, Keiichi Omote, Masaki Toriyabe
1Department of Anesthesiology, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan. kawamata@sapmed.ac.jp
Abstract:
We carried out experiments using a microdialysis method to determine whether activation of 5-HT(3) receptors increases the concentration of GABA in the dorsal horn of the spinal cord. Intrathecal perfusion of a selective 5-HT(3) receptor agonist, 1-phenylbiguanide (0.3, 1.0, and 3.0 mM) dose-dependently, increased GABA concentration, while concentrations of glutamate and glycine were not changed. The concentration of aspartate was increased only by 3.0 mM of 1-phenylbiguanide. Our results have provided direct evidence that activation of 5-HT(3) receptors evokes GABA release in the spinal dorsal horn, possibly producing analgesia.
More Related Videos
07:51Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
Published on: November 14, 2014
07:16Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
Published on: August 16, 2018
Related Concept Videos
Ligand-Gated Ion Channel Receptor: Gating Mechanism
G-Protein Gated Ion Channels
Sensory organs,...
Activation and Inactivation of G Proteins
GPCRs Regulate Adenylyl Cylase Activity
Two...
Parasympathetic Signaling
The effects of...
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...