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Problems connected with band-broadening in size-exclusion chromatography with dual detection
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. netopil@imc.cas.cz
Journal of Biochemical and Biophysical Methods
|July 2, 2003
Abstract:
The sources of band-broadening (axial dispersion) in size-exclusion chromatography (SEC) of polymers and its influence on data obtained using dual detection of concentration and molecular weight are reviewed. Special attention is paid to the combination of a multiangle light-scattering photometer and a differential refractometer as detectors. The local polydispersity is discussed in the relation to the band-broadening as well as to heterogeneity of the polymer with respect to molecular weight and hydrodynamic volume.