Related Experiment Video
Updated: Aug 8, 2026

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
Published on: January 8, 2013
Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of
M Pilar Tarazona1, Enrique Saiz
1Departamento de Química Física, Universidad de Alcalá, 28871 Alcalá de Henares, Spain. mpilar.tarazona@uah.es
Abstract:
Size-exclusion chromatography (SEC) with dual detection, i.e., employing refractive index (RI) and multiangle light-scattering (MALS) detectors, has been applied to study the solution properties of two very different polymer-solvent systems at 25 degrees C: poly(N-vinylcarbazole) (PVCz) in an organic solvent THF that is a very good solvent and a system under theta conditions that is formed by polyvinylpyrrolidone (PVP) in water containing a 0.1 M concentration of NaNO(3). In both cases, the analysis of a single highly polydisperse sample obtained by free radical polymerization is enough for obtaining molecular weight and radius of gyration calibration curves, molecular weight distributions (MWD) (and thus, molecular weight averages), molecular dimensions, scaling laws coefficients and unperturbed dimensions. Extrapolation to theta conditions produces values of the characteristic ratio of the unperturbed dimensions C(n)=
Related Concept Videos
Freezing Point Depression and Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Chemical Equilibria: Systematic Approach to Equilibrium Calculations
The first step is to identify all the chemical reactions involved, The...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Determination of Molar Masses of Polymers II

