Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosensing under an applied voltage using optical waveguide lightmode spectroscopy.

Michelle A Brusatori1, Paul R Van Tassel

  • 1Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202, USA.

Biosensors & Bioelectronics
|July 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polyelectrolyte complexation with biofunctionalized multivalent ions: Coarse-grained model and isothermal titration calorimetry experiment.

The Journal of chemical physics·2026
Same author

Polyelectrolyte Influence on Beta-Hairpin Peptide Stability: A Simulation Study.

The journal of physical chemistry. B·2022
Same author

Equilibrium state model for surfactants in oils: Colloidal assembly and adsorption.

Journal of colloid and interface science·2022
Same author

A "New Radiation"-shining light on cancer.

Cancer metastasis reviews·2020
Same author

Weak polyelectrolyte-based multilayers via layer-by-layer assembly: Approaches, properties, and applications.

Advances in colloid and interface science·2020
Same author

Applications of Raman spectroscopy in cancer diagnosis.

Cancer metastasis reviews·2018
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
See all related articles

Applying a DC voltage enhances biomacromolecule adsorption in biosensors. This method uses optical waveguide lightmode spectroscopy (OWLS) to continuously measure adsorption, improving biosensing applications.

Area of Science:

  • Biosensing and biomaterial interfaces
  • Applied physics and electrochemistry

Background:

  • Biosensor fabrication and detection often require controlled biomacromolecule immobilization.
  • Optical waveguide lightmode spectroscopy (OWLS) is a sensitive technique for monitoring surface binding events.

Purpose of the Study:

  • To develop and validate a method for directly measuring polyelectrolyte adsorption onto an electrode under an applied DC voltage.
  • To investigate the effect of applied potential on the adsorption of specific biomacromolecules, human serum albumin and cytochrome c.

Main Methods:

  • Utilized optical waveguide lightmode spectroscopy (OWLS) with an indium tin oxide (ITO) coated silicon titanium oxide (STO) waveguiding film as the working electrode.
  • Applied a DC voltage across the ITO electrode to influence the interfacial double layer and protein adsorption.

Related Experiment Videos

  • Employed a simplified optical model combining STO and ITO layers for accurate refractive index change detection.

    • Main Results:

    • An applied potential significantly increased the effective refractive index, attributed to interfacial double layer charging and potential oxidation.
    • Further increases in refractive index confirmed solution adsorption onto the ITO electrode.
    • Adsorption of human serum albumin and horse heart cytochrome c was significantly enhanced (over 1 V potential) due to complementary charge interactions.

    Conclusions:

    • Applied DC voltage can effectively control and enhance biomacromolecule adsorption in biosensing.
    • The OWLS method with a combined STO-ITO optical model provides accurate, continuous monitoring of potential-influenced adsorption.
    • Understanding charge-based interactions is crucial for optimizing biosensor performance through electrical potential control.