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Updated: Aug 17, 2026

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
Published on: May 26, 2011
Studies on Bacillus stearothermophilus. Part III. Transformation of testosterone
S Al-Awadi1, M Afzal, S Oommen
1Department of Biological Sciences, Biochemistry Program, Faculty of Science, Kuwait University, P.O. Box 5969, 13060 Safat, Kuwait. sameera@kuc0l.kuniv.edu.kw
Abstract:
Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabolites included 6 alpha-hydroxyandrost-4-en-3,17-dione, 6 beta-hydroxyandrost-4-en-3,17-dione, 6 alpha-hydroxytestosterone, and 6 beta-hydroxytestosterone. These metabolites were purified by TLC and HPLC followed by their identification using (1)H- and (13)C-NMR and other spectroscopic data.
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