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Related Experiment Videos

[A practical volume controlled bleeding method in animal hemorhagic shock model].

Hakan Güven1, Murat Hökelek

  • 1Ondokuz Mayis Universitesi Tip Fakültesi, Ilk ve Acil Yardim AD, Kurupelit/Samsun, Turkey. hakang@omu.edu.tr

Ulusal Travma Ve Acil Cerrahi Dergisi = Turkish Journal of Trauma & Emergency Surgery : TJTES
|July 2, 2003
PubMed
Summary

The traditional bleeding method for hemorrhagic shock models is difficult to control. A reversely connected infusion pump offers a practical solution for precise blood withdrawal, improving research accuracy.

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Area of Science:

  • Physiology
  • Animal Models
  • Surgical Techniques

Context:

  • Hemorrhagic shock research relies on accurate blood loss simulation.
  • The conventional bleeding method using syringes presents challenges in controlling blood withdrawal rate and volume.
  • Inconsistent bleeding can lead to experimental variability and animal mortality.

Purpose:

  • To introduce a novel, practical method for controlled blood withdrawal in animal models.
  • To overcome the limitations of traditional bleeding techniques in establishing precise blood loss parameters.
  • To enhance the reliability of hemorrhagic shock models for research.

Summary:

  • This report details a method utilizing a reversely connected infusion pump for controlled blood withdrawal.
  • This technique allows for predetermined bleeding volumes and rates, addressing the variability of manual syringe methods.

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  • The described approach ensures consistent exsanguination, crucial for reproducible experimental outcomes.

    • Impact:

    • Provides a more reliable and reproducible method for inducing controlled hemorrhagic shock in laboratory animals.
    • Facilitates accurate studies on acute blood loss, pathophysiology, and metabolic responses.
    • Reduces experimental error and potential mortality associated with imprecise bleeding techniques.