A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside
1Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
We developed a conceptually new strategy for the coupling method in the phosphoramidite method without protection of nucleoside bases. This strategy, called "proton-block method", significantly reduced side reactions compared with the previous methods.
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