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Updated: Jul 25, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis
T Sakamoto1, A Mahara, R Iwase
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Abstract:
Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.
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