Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virus-like particles with reverse transcriptase activity associated with Kawasaki disease.

C Y Lin1, I C Chen, T I Cheng

  • 1Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, Republic of China.

Journal of Medical Virology
|November 1, 1992
PubMed
Summary

Researchers found virus-like particles with reverse transcriptase activity in Kawasaki disease (KD) patients, suggesting a potential retroviral link to this illness.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cells resident to precision templated 40-µm pore scaffolds generate small extracellular vesicles that affect CD4<sup>+</sup> T cell phenotypes through regulatory TLR4 signaling.

Acta biomaterialia·2023
Same author

Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children.

Andrology·2016
Same author

Improving access to antiretrovirals in rural South Africa - a call to action.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2015
Same author

Pancreatic adenocarcinoma upregulated factor, a novel endothelial activator, promotes angiogenesis and vascular permeability.

Oncogene·2012
Same author

Immunomodulatory effects of mixed hematopoietic chimerism: immune tolerance in canine model of lung transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2009
Same author

Kinetics of microbial hydrogenation of free linoleic acid to conjugated linoleic acids.

Journal of applied microbiology·2008

Area of Science:

  • Virology
  • Immunology
  • Pediatric Infectious Diseases

Background:

  • Kawasaki disease (KD) is an acute febrile illness of unknown etiology primarily affecting young children.
  • Previous research has explored infectious agents, including viruses, as potential triggers for KD.
  • The role of retroviruses in KD pathogenesis remains largely uninvestigated.

Purpose of the Study:

  • To investigate the potential etiologic agent, specifically retroviruses, associated with Kawasaki disease.
  • To establish a cocultivation system to detect virus-like particles and associated enzymatic activity in KD patients.

Main Methods:

  • Cocultivation of concanavalin A-stimulated lymphoblastoid cells from retrovirus-seronegative individuals with cells from KD patients, other viral infection patients, and controls.

Related Experiment Videos

  • Measurement of reverse transcriptase (RT) activity, electron microscopy for virus-like particles, SDS-PAGE for viral products, and RT-PCR for retroviral gene detection.

    • Main Results:

    • Significantly higher RT activity was observed in KD patients compared to controls (P < 0.0001).
    • Virus-like particles (80-100 nm) and a 31.4 kDa viral product were identified in KD patient samples.
    • Retrovirus-specific DNA sequences were detected in KD patients using RT-PCR.

    Conclusions:

    • The findings suggest an association between virus-like particles with reverse transcriptase activity and Kawasaki disease.
    • These results point towards a potential retroviral etiology for Kawasaki disease.
    • Further investigation is warranted to confirm the role of retroviruses in KD pathogenesis.