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Antibodies in proteomics II: screening, high-throughput characterization and downstream applications
Andrew Bradbury1, Nileena Velappan, Vittorio Verzillo
1B Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. amb@lanl.gov
Trends in Biotechnology
|July 3, 2003
Summary
Improving antibody use for high-throughput characterization is key. Display technologies and novel methods offer new avenues for robust proteomic data generation, moving beyond traditional antibody approaches.
Area of Science:
- Biotechnology
- Proteomics
- Immunology
Background:
- Current antibody characterization methods are often limited in throughput.
- Focus has been on well-characterized commercial antibodies, hindering broader application.
Purpose of the Study:
- To explore improvements in antibody use and selection for high-throughput characterization.
- To adapt existing and novel methods for enhanced proteomic data generation.
Main Methods:
- Utilizing antibody fragments from display technologies with standard protocols (immunoprecipitation, western blotting, immunofluorescence).
- Exploring novel approaches like antibody chips and intracellular immunization.
- Adapting protocols for increased reliability and robustness.
Main Results:
- Standard protocols can be adapted for antibody fragments from display technologies.
- Novel methods combined with adapted protocols can yield useful proteomic data.
- Adaptation is necessary for achieving higher throughput.
Conclusions:
- Broader use of antibody fragments and display technologies is essential for high-throughput characterization.
- Novel approaches and protocol optimization are critical for advancing proteomic analysis.
- Traditional methods alone are insufficient for future throughput demands.