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Updated: Sep 21, 2026
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Published on: July 10, 2014
[Extraction and purification of porcine amelogenin and preparation for the polyclonal amelogenin antibody]
Chenrong Xu1, Ailing Yang, Jincai Zhang
1Department of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
To prepare the polyclonal antibody to amelogenin.
Methods:
The fetal porcine dental enamel was collected. Enamel matrix protein was extracted in 4M guanidine HCl (pH 7.4) with protease inhibitors present. Polyacrylamide gel filtration was included to isolate amelogenin from the initial dissociated extraction. The purified amelogenin conjugated with or without complete/incomplete Freund's adjuvant was then used to immunize the rabbits subcutaneously or intravenously. The specific IgG antibody was further purified by DE-52 cellulose. The working concentration of IgG antibody was determined through ELISA test.
Results:
The Gel filtration showed that amelogenin components is at molecular weights of 15 kD and 13 kD apparently, which was consistent with those described before. The ELISA results showed that the working concentration for IgG was 1:1000.
Conclusion:
The antibody prepared in this study can be used for the detection of amelogenin.
