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[The isolation of ribosomes from Acholeplasma and the study of their physicochemical characteristics]
Abstract:
Ribosomes are produced from Acholeplasma laidlawii PG-8 and A. laidlawii var. granulum str. 118. It is proved as possible to use the standard method of differential centrifugation for isolation of mollicute ribosomes. The physico-chemical properties of acholesplasma ribosomes are studied. The protein content for A. laidlawii PG-8 amounted to 39.6%, rRNA content--60.4% and for A. laidlawii var. granulum--39.1 and 60.8%, respectively. The RNA: protein ratio equals 1.5:1, the ratio of optic density indicates at 260 and 280 nm--2, that is peculiar to treated preparations of ribosomes. Sedimentation coefficient of acholeplasma ribosomes is 70S. The produced preparations of acholeplasma ribosomes can be used subsequently for creating the system of translation in vitro to study the biosynthesis processes of mollicute protein.
Insights
Researchers successfully isolated and characterized mollicute ribosomes from Acholeplasma laidlawii strains. These purified ribosomes are suitable for in vitro studies of mollicute protein biosynthesis.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Mollicutes, a unique class of bacteria, lack cell walls.
- Understanding their cellular machinery, like ribosomes, is crucial for studying their biology.
Purpose of the Study:
- To isolate and characterize ribosomes from Acholeplasma laidlawii.
- To assess the suitability of these ribosomes for in vitro translation studies.
Main Methods:
- Differential centrifugation was employed for mollicute ribosome isolation.
- Physicochemical properties, including protein and rRNA content, were analyzed.
Main Results:
- Ribosomes were successfully isolated from A. laidlawii PG-8 and A. laidlawii var. granulum.
- Protein content was approximately 39%, with rRNA content around 60%.
- A sedimentation coefficient of 70S was determined for these ribosomes.
Conclusions:
- Standard differential centrifugation is effective for isolating mollicute ribosomes.
- Acholeplasma laidlawii ribosomes are suitable for establishing in vitro translation systems.
- This work facilitates further research into mollicute protein biosynthesis.