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Related Experiment Videos

Construction and production of fluorescent papillomavirus-like particles.

S Peng1, J Zhou, I H Frazer

  • 1Department of Pediatrics, Xiehe Hospital, Tongji Medical University, Wuhan 430022.

Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao
|July 5, 2003
PubMed
Summary

Researchers developed fluorescent bovine papillomavirus (BPV) virus-like particles (VLPs) using green fluorescent protein (GFP). These GFP-tagged BPV VLPs can be used to study papillomavirus-cell interactions, overcoming limitations in traditional cell culture systems.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Virology

Background:

  • The green fluorescent protein (GFP) from Aequorea victoria is a widely used reporter protein due to its in vivo fluorescence.
  • Investigating the papillomavirus life cycle is challenging due to the lack of conventional cell culture systems.
  • Bovine papillomavirus (BPV) type 1 is a model system for studying papillomaviruses.

Purpose of the Study:

  • To construct chimeric BPV type 1 virus-like particles (VLPs) incorporating GFP.
  • To assess the feasibility of using fluorescent VLPs for studying papillomavirus-cell interactions.
  • To develop a novel tool for analyzing papillomavirus entry and lifecycle.

Main Methods:

  • Construction of chimeric BPV type 1 VLPs by inserting GFP into the L2 protein (aa 88) or creating a GFP/L2 fusion protein.

Related Experiment Videos

  • Assembly of fluorescent VLPs in vitro.
  • Analysis of VLP/cell interaction using CV-1 cells and Fluorescence-Activated Cell Sorting (FACS) assay.

    • Main Results:

    • Fluorescent VLPs were successfully assembled using either GFP-inserted L2 or a GFP/L2 fusion protein.
    • The incorporation of GFP did not disrupt the structural integrity of the BPV1 VLPs.
    • In vitro, fluorescent VLPs demonstrated binding to CV-1 cells, detectable by FACS analysis.

    Conclusions:

    • Green fluorescent protein (GFP) can be successfully incorporated into bovine papillomavirus type 1 (BPV1) virus-like particles (VLPs) without compromising VLP structure.
    • These fluorescent BPV1 VLPs represent a valuable tool for investigating papillomavirus-host cell interactions.
    • The developed method offers a promising approach to overcome limitations in studying papillomavirus biology.