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Updated: Aug 1, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Synthesis of acyclothymidine triphosphate and alpha-P-boranotriphosphate and their substrate properties with
Ping Li1, Mikhail Dobrikov, Hongyan Liu
1Department of Chemistry, Box 90346, Duke University, Durham, North Carolina 27708-0346, USA.
Abstract:
[reaction: see text] The first example of an acyclonucleoside alpha-P-boranotriphosphate has been synthesized via a phosphoramidite approach in a one-pot reaction with good yield. The presence of the alpha-P-BH(3) in 5b results in a 9-fold increase in efficiency of incorporation by MMLV retroviral reverse transcriptase relative to non-boronated 5a in pre-steady-state conditions. The preliminary results indicate that acyclonucleoside alpha-P-boranotriphosphates may have promising applications as a probe of enzyme mechanisms and in the design of new antiviral drugs.
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