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Published on: February 3, 2013
Immunofluorescence detection of the vimentin epitope in chromatin structures of cell nuclei and chromosomes
H Fidlerová1, V Sovová, I Krekule
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Abstract:
We describe immunofluorescence detection of the vimentin epitope, recognized by monoclonal antibody VI-01, in chromatin structures of eukaryotic cell nuclei and chromosomes. The approach used is based on increased sensitivity of 5-bromodeoxyuridine (BrdU)-substituted DNA to UV irradiation-induced crosslinking of DNA with proteins in vivo, by which the proteins interacting with chromosomal DNA can be immunovisualized in situ.
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