Evidence for chimeric sequences formed during random arbitrarily primed PCR
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Updated: Sep 23, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
In Seop Chang1, Jimmy D Ballard, Lee R Krumholz
1Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019-0245, USA.
Abstract:
Chimeric sequences were observed to occur abundantly (48% of clones) during random arbitrarily primed polymerase chain reaction (RAP-PCR) experiments designed to examine differential expression of genes involved in metal resistance in sulfate-reducing bacteria (SRB). Some of the chimeric sequences were composed of sequence from a gene differentially expressed under the imposed conditions and a sequence of the 16S or 23S rRNA gene. The remainder were composed of two rRNA sequences. Experiments using PCR and genomic sequence analysis showed that the chimeric sequences were not due to a genetic mutation (e.g., recombination, transposition). As RAP-PCR has been widely used to identify differentially expressed genes, this observation may aid in our interpretation of RAP-PCR data.
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