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Related Experiment Videos

Chronic atrial fibrillation does not further decrease outward currents. It increases them.

Wen Dun1, Parag Chandra, Peter Danilo

  • 1Department of Pharmacology, Center for Molecualr Therapeutics, Columbia College of Physicians and Surgeons, 630 W. 168th St., New York, NY 10032, USA.

American Journal of Physiology. Heart and Circulatory Physiology
|July 5, 2003
PubMed
Summary
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Electrical remodeling in atrial fibrillation (AF) differs based on pacing versus sustained AF. Rapid atrial pacing alone causes distinct changes in transient outward potassium current (Ito) compared to chronic AF.

Area of Science:

  • Cardiology
  • Electrophysiology
  • Molecular Biology

Background:

  • Rapid atrial pacing can induce electrical remodeling, leading to atrial fibrillation (AF).
  • AF itself can further remodel atrial electrophysiology, potentially sustaining the arrhythmia.
  • Previous studies noted variability in AF duration in dogs subjected to rapid atrial pacing.

Purpose of the Study:

  • To investigate the differences in electrical remodeling caused by rapid atrial pacing versus chronic AF.
  • To compare the changes in transient outward potassium current (Ito) and sustained outward potassium current (Isus) in atrial cells from dogs with different AF durations.

Main Methods:

  • Isolation of right atrial cells from control dogs, dogs with chronic AF (cAF), and dogs with non-sustained AF (nAF).

Related Experiment Videos

  • Whole-cell voltage-clamp techniques to measure transient outward (Ito) and sustained outward (Isus) potassium currents.
  • Analysis of Ito density, voltage dependence of inactivation, decay kinetics, and recovery from inactivation.

    • Main Results:

    • nAF cells showed a 69% reduction in Ito density compared to control cells.
    • cAF cells had significantly different Ito density compared to both control and nAF cells, with a smaller reduction than nAF cells.
    • Ito remodeling in nAF cells differed from cAF cells, with altered inactivation and decay kinetics in cAF cells.

    Conclusions:

    • Both rapid atrial pacing and chronic AF lead to reduced Ito, but the remodeling patterns differ.
    • Ito remodeling in cAF dogs appears to be influenced by AF itself.
    • Ito remodeling in nAF dogs is likely a consequence of rapid pacing in the absence of sustained AF.