Detection of Legionella pneumophila by real-time PCR for the mip gene
Deborah A Wilson1, Belinda Yen-Lieberman, Udo Reischl
1Cleveland Clinic Foundation, Cleveland, Ohio, USA.
Journal of Clinical Microbiology
|July 5, 2003
Abstract:
A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.
