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Overexpression and purification of DNA topoisomerase I from yeast

M A Bjornsti1, J Fertala

  • 1Department of Pharmaceutical Science, St. Jude Children's Research Hospital, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2003
PubMed
Abstract

No abstract available in PubMed .

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Related Concept Videos

DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...