Related Experiment Video
Updated: Sep 23, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
Published on: September 17, 2020
SIR-dependent repression of non-telomeric genes in Saccharomyces cerevisiae?
Uta Marchfelder1, Katja Rateitschak, Ann E Ehrenhofer-Murray
1Otto-Warburg-Laboratories, Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany.
Abstract:
The proteins Sir2, Sir3 and Sir4 repress transcription of the silent mating-type loci HML and HMR and of reporter genes inserted at telomeres. Previous microarray analyses suggested that additional non-telomeric genes exist which are repressed by the Sir proteins. In this study, we tested the expression of 12 such genes by Northern analysis and RT-PCR. However, we were unable to verify their SIR-dependent regulation, which suggests that SIR-mediated repression may be restricted to the known repressed regions.
Related Concept Videos
Replicative Cell Senescence
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Telomeres and Telomerase
Gene Regulation During Sporulation
Constitutive and Regulated Gene Expression
Repressible Operon: trp Operon

