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Updated: Sep 23, 2026

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Published on: May 1, 2012
Covalent attachment of DNA to glass supports using a new silane coupling agent and chemiluminescent detection
1Institute of Environmental Medicine, Tongji Medical University, Wuhan 430030.
Abstract:
A new kind of silane coupling agent, N-(beta-aminoethyl)-gamma-aminopropyl triethoxysilane, was used for DNA direct attachment on the surfaces of glass supports, then the immobilized DNA was hybridized with horseradish peroxidase (HRP)-labeled probe, and detected by using enhanced chemiluminescent method. In comparison with gamma-aminopropyl triethoxysilane, the detection limits (S/N) of DNA were 10 pg and 75 pg respectively. Several experimental conditions of DNA attach-ING to glass supports were investigated, and the system of hybridization of nucleic acid on the surfaces of glass supports was developed.
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