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[Lymphocytograms in relation to storage time of CPDA1 whole blood concentrates]
I Menke-Möllers1, H Burrichter, K Schiene
1Institut für Transfusionsmedizin, Universität zu Köln, BRD.
Immunocompetent cells have been shown to be effective in immunosuppression after blood transfusion. The analysis of leukocytes by electronic cell counter and of lymphocyte subsets by flow cytometry in stored whole blood revealed a decay of granulocytes within the first day of storage. T-lymphocyte subpopulations and natural killer cells continuously decrease over the time of storage, whereas B lymphocytes are very stable during the first 2 weeks. After 4 weeks lymphocyte subsets had decreased by the following values: total lymphocytes 46.0%, CD3+ 78.3%, CD4+ 80.1%, CD8+ 81.3%, CD19+ 65.9%, CD16/56+ 74.8% and CD3/DR+ 89.5%. Therefore leukocyte removal should be achieved as soon as possible after collection.
Immunocompetent cells have been shown to be effective in immunosuppression after blood transfusion. The analysis of leukocytes by electronic cell counter and of lymphocyte subsets by flow cytometry in stored whole blood revealed a decay of granulocytes within the first day of storage. T-lymphocyte subpopulations and natural killer cells continuously decrease over the time of storage, whereas B lymphocytes are very stable during the first 2 weeks. After 4 weeks lymphocyte subsets had decreased by the following values: total lymphocytes 46.0%, CD3+ 78.3%, CD4+ 80.1%, CD8+ 81.3%, CD19+ 65.9%, CD16/56+ 74.8% and CD3/DR+ 89.5%. Therefore leukocyte removal should be achieved as soon as possible after collection.