[Permselect--a new substance for leukocyte elimination].

Area of Science:

• Biomaterials Science
• Cell Biology
• Hematology

Background:

• Leukocyte contamination in blood products poses risks.
• Effective methods for leukocyte depletion are crucial for transfusion safety.
• Plant-derived materials offer potential for novel biomedical applications.

Purpose of the Study:

• To evaluate the efficacy of Permselect particles for leukocyte depletion from blood.
• To compare Permselect's performance against commercial leukocyte removal filters.
• To assess the impact of Permselect filtration on erythrocyte viability and stability.

Main Methods:

• Permselect particles were prepared from Chenopodium album L. biomass.
• Leukocyte binding efficiency was quantified using fresh blood, preserved whole blood, and red blood cell concentrates.
• Filtration experiments compared Permselect with cotton and cellulose acetate filters.
• Erythrocyte vitality and storage stability were assessed post-filtration.

Main Results:

• Permselect demonstrated very high efficiency, removing 99-100% of leukocytes from various blood preparations.
• Permselect exhibited superior leukocyte binding compared to commercial filter materials.
• Erythrocyte vitality and stability were unaffected by Permselect filtration.
• The pectin-rich surface of Permselect particles is implicated in leukocyte binding.

Conclusions:

• Permselect is a highly effective and safe material for leukocyte depletion in blood products.
• Plant-derived cell wall materials represent a promising alternative to synthetic filters.
• Further research into the mechanism of leukocyte-cell wall interaction is warranted.